XCeloSeq® Targeted cfDNA Enrichment Kits 

NGS Library Preparation kits for cancer detection from liquid biopsy.

The XCeloSeq Targeted cfDNA Enrichment Kits generate high quality sequencing libraries for cancer detection from liquid biopsy (cfDNA) samples or FFPE-preserved material.

 

ENHANCED ERROR CORRECTION

 XCeloSeq-generated sequencing libraries contain unique molecular identifiers (UMI) and a unique protocol design, which combine for enhanced error suppression and sensitivity.

STREAMLINED AND FAST WITH HIGHEST SAMPLE RETENTION

 XCeloSeq protocols have been designed to be completed within 1 day with the fewest bead purifications and shortest hands-on times.

ENHANCED LIBRARY COMPLEXITY

All XCeloSeq products use the ATOM-Seq® capture chemistry which is built to be uniquely suited for use with liquid biopsy and FFPE material, delivering unrivalled library complexity

Sensitivity:

Allele frequency: 0.1%

Input Amount*:

Recommended: 5-50ng

Minimum: 1ng

Protocol Time:

Total: 5h 40m

Hands-on: 1h 20m

The XCeloSeq Targeted cfDNA Workflow

Using the ATOM-Seq DNA capture method, Unique Molecular Identifiers (UMIs) and universal adapter sequences are incorporated directly into available 3' ends of every original sample molecule. Then, two rounds of target-specific PCR generate sequencing-ready libraries.

ATOM-Seq DNA Capture

Hands-on Time: 10 minutes

 Incubation Time: 1 hour and 45 minutes

Starting materials (cfDNA, FFPE DNA or high quality genomic DNA) are combined with the Adapter Template Oligo (ATO).

Incorporation of universal priming site and Unique Molecular Indicator directly onto original starting molecule.

target_cfDNA_workflow_1.png
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Two rounds of Nested,

Target-Specific PCR

Hands-on Time: 1 hour and 10 minutes
Incubation Time: 2 hour and 35 minutes

Multiplexed target-specific primers are paired against a universal primer which binds the extension that was added by the ATO reaction. Together these amplify target mutation hotspots by PCR.

A second round of target-specific PCR using a nested primer maximises on-target rate of sequencing, and incorporates dual-index sample barcodes (i5 and i7).

Sequencing-ready library in 5 hours, 40 minutes.

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The XCeloSeq Targeted cfDNA Enrichment Product Range:

All Targeted cfDNA Enrichment Kits use a common Instructions For Use document, available here.

Please also see the relevant product insert(s) for kit-specific information and protocol steps, given below.

XCeloSeq Pan Cancer cfDNA Kit

A comprehensive targeted hotspot panel for somatic mutations in liquid biopsy or FFPE samples, for a variety of cancer types.

Product code: SEQ002

View Product Insert

Gene Targets: 100

Reactions per kit: 8

Targeting Primers: 1147

View target genes

Please wait - retrieving target list

* Whole Coding Region Coverage

XCeloSeq Colon Cancer cfDNA Kit

A targeted hotspot panel for colon cancer-specific somatic mutations in liquid biopsy or FFPE samples.

Product code: SEQ009

View Product Insert

Gene Targets: 23

Reactions per kit: 8

Targeting Primers: 315

View target genes

Please wait - retrieving target list

* Whole Coding Region Coverage

XCeloSeq Lung Cancer cfDNA Kit

A targeted hotspot panel for lung cancer-specific somatic mutations in liquid biopsy or FFPE samples.

Product code: SEQ010

View Product Insert

Gene Targets: 17

Reactions per kit: 8

Targeting Primers: 300

View target genes

Please wait - retrieving target list

* Whole Coding Region Coverage

XCeloSeq Breast Cancer cfDNA Kit

A targeted hotspot panel for breast cancer-specific somatic mutations in liquid biopsy or FFPE samples.

Product code: SEQ011

View Product Insert

Gene Targets: 12

Reactions per kit: 8

Targeting Primers: 245

View target genes

Please wait - retrieving target list

* Whole Coding Region Coverage

XCeloSeq Indexing Kits (SEQ005)

Dual indexing primers to enable sample multiplexing. Each kit contains either eight i5 or eight i7 sample index primers.

Kits can be purchased separately to suit your multiplexing requirements. 

These primers can be used as substitutes for the default pair of sample indexing primers included in any XCeloSeq Library preparation kit, including XCeloSeq Targeted cfDNA kits.

Dual indexing primers to enable sample multiplexing. Each kit contains either eight i5 or eight i7 sample index primers.

Kits can be purchased separately to suit your multiplexing requirements. 

* Higher quantities within this range will improve maximum sensitivity, recommended maximum is 50 ng. The product supports capture with down to 1.0 ng of cfDNA, however this will lead to reduced sensitivity. Enzymatically fragmented FFPE is also supported as an alternative starting material.

For more information or for sales equniries, please contact us here or at info@genefirst.com