XCeloSeq® Targeted cfDNA Enrichment Kits
NGS Library Preparation kits for cancer detection from liquid biopsy.
The XCeloSeq Targeted cfDNA Enrichment Kits generate high quality sequencing libraries for cancer detection from liquid biopsy (cfDNA) samples or FFPE-preserved material.
ENHANCED ERROR CORRECTION
XCeloSeq-generated sequencing libraries contain unique molecular identifiers (UMI) and a unique protocol design, which combine for enhanced error suppression and sensitivity.
STREAMLINED AND FAST WITH HIGHEST SAMPLE RETENTION
XCeloSeq protocols have been designed to be completed within 1 day with the fewest bead purifications and shortest hands-on times.
ENHANCED LIBRARY COMPLEXITY
All XCeloSeq products use the ATOM-Seq® capture chemistry which is built to be uniquely suited for use with liquid biopsy and FFPE material, delivering unrivalled library complexity
Sensitivity:
Allele frequency: ≥0.1%
Input Amount*:
Recommended: 5-50ng
Minimum: 1ng
Protocol Time:
Total: 5h 40m
Hands-on: 1h 20m
The XCeloSeq Targeted cfDNA Workflow
Using the ATOM-Seq DNA capture method, Unique Molecular Identifiers (UMIs) and universal adapter sequences are incorporated directly into available 3' ends of every original sample molecule. Then, two rounds of target-specific PCR generate sequencing-ready libraries.
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ATOM-Seq DNA Capture
Hands-on Time: 10 minutes
Incubation Time: 1 hour and 45 minutes
Starting materials (cfDNA, FFPE DNA or high quality genomic DNA) are combined with the Adapter Template Oligo (ATO).
Incorporation of universal priming site and Unique Molecular Indicator directly onto original starting molecule.
Two rounds of Nested,
Target-Specific PCR
Hands-on Time: 1 hour and 10 minutes
Incubation Time: 2 hour and 35 minutes
Multiplexed target-specific primers are paired against a universal primer which binds the extension that was added by the ATO reaction. Together these amplify target mutation hotspots by PCR.
A second round of target-specific PCR using a nested primer maximises on-target rate of sequencing, and incorporates dual-index sample barcodes (i5 and i7).
Sequencing-ready library in 5 hours, 40 minutes.
The XCeloSeq Targeted cfDNA Enrichment Product Range:
All Targeted cfDNA Enrichment Kits use a common Instructions For Use document, available here.
Please also see the relevant product insert(s) for kit-specific information and protocol steps, given below.
Please note that an XCeloSeq UDI Kit (containing unique premixed i5 and i7 indexing primers) must be purchased separately to use any of the library preparation kits listed on this page. Please see below for more information.
View target genes
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* Whole Coding Region Coverage
View target genes
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* Whole Coding Region Coverage
View target genes
Please wait - retrieving target list
* Whole Coding Region Coverage
View target genes
Please wait - retrieving target list
* Whole Coding Region Coverage
XCeloSeq Unique Dual Indexing Sets (IDX1-01 to IDX1-12)
The XCeloSeq Unique Dual Indexing (UDI) Sets allow for multiplexing of up to 96 different samples each with a unique combination of i5 and i7 indexes for next-generation sequencing on Illumina sequencing platforms. All index oligos undergo rigorous quality control procedures including generation and sequencing of indexed libraries to ensure high quality performance with all compatible XCeloSeq Library Preparation Kits.
When purchasing UDI Sets for use in combination with any of the XCeloSeq Targeted cfDNA Enrichment Kits up to 96 samples can be multiplexed together when purchasing all 12 Sets. Each UDI combination contains enough for 8 samples, for a total of 64 samples for every UDI Set.
At least one UDI Set must be purchased with XCeloSeq Targeted cfDNA Enrichment Kits.
Click Here to download product information for ordering, multiplexing, and sample requirements.
* Higher quantities within this range will improve maximum sensitivity, recommended maximum is 50 ng. The product supports capture with down to 1.0 ng of cfDNA, however this will lead to reduced sensitivity. Enzymatically fragmented FFPE is also supported as an alternative starting material.
For more information or for sales equniries, please contact us here or at info@genefirst.com