XCeloSeq Pan Cancer cfDNA Kit V2
®
NGS Library Preparation kit for use with cell-free DNA from liquid biopsy
Home > ATOM-Seq Capture Chemistry > Cell-free DNA Enrichment Workflow > XCeloSeq Pan Cancer cfDNA Kit V2
Use ATOM-Seq to capture cell-free DNA and enrich for mutation hotspots across 100 of the most frequently mutated genes across cancer for detection of even the rarest mutations.
Product Details
Specifications
Product Code
SEQ030
Gene Targets
100
Targeting Primers
Pool 1: 577, Pool 2: 570
Supported Input Material
Cell-free DNA
Recommended Input Quantity
Recommended: 30 - 50 ng
Minimum: 1 ng
Larger quantities will improve maximum sensitivity
Required time
Hands-on: 1.5 hours
Total: 6 hours
Platform compatability
Illumina
Target Genes
Targets mutation hotspots across 100 genes most frequently mutated across cancers
ABL1
BRCA1
CHEK2
ESR1
GNAQ
KEAP1
MSH2
NTRK3
RHOA
SMO
AKT1
BRCA2
CSF1R
EZH2
GNAS
KIT
MSH6
PDGFRA
RIT1
SRC
ALK
CASP8
CTNNB1
FBXW7
HNF1A
KLF5
MTOR
PIK3CA
RNF43
STK11
AMER1
CCND1
DDR2
FGFR1
HRAS
KRAS
MYC
PTCH1
ROS1
TCF7L2
APC
CCND2
DMD
FGFR2
IDH1
MAP2K1
NF1
PTEN
SETD2
TP53*
AR
CCND3
EGFR
FGFR3
IDH2
MAP2K2
NFE2L2
PTPN11
SF3B1
TSC1
ARAF
CDH1
EP300
FGFR4
JAK2
MET
NOTCH1
RAF1
SMAD2
TSC2
ARID1A
CDK4
ERBB2
FLT3
JAK3
MGA
NPM1
RB1
SMAD4
U2AF1
ATM
CDK6
ERBB3
GATA3
KDM6A
MLH1
NRAS
RBM10
SMARCA4
VHL
BRAF
CDKN2A
ERBB4
GNA11
KDR
MPL
NTRK1
RET
SMARCB1
ZFP36L2
*Whole coding region coverage (NM_000546)
Benefits
Capture more of your patient sample with ATOM-Seq which captures all single stranded, double-stranded or nicked DNA of any length.
Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations
Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases
Unique Dual Indexes
Unique Dual Indexes (UDIs) are required to be purchased and are sold separately
Please contact us to discuss additional index options.
Resources
Product guide and protocol
For Research Use Only (RUO).
Not for use in diagnostic procedures. Not intended to be used for treatment of human or animal diseases.