XCeloSeq Lung Cancer cfDNA Kit V2
®
NGS Library Preparation kit for use with cell-free DNA from liquid biopsy
Use ATOM-Seq to capture cell-free DNA and enrich for mutation hotspots across 17 genes frequently mutated in lung cancer detection of the even the rarest mutations.
Product Details
Specifications
Product Code
SEQ032
Gene Targets
17
Targeting Primers
Pool 1: 150, Pool 2: 146
Supported Input Material
Cell-free DNA
Recommended Input Quantity
Recommended: 30 - 50 ng
Minimum: 1 ng
Larger quantities will improve maximum sensitivity
Required time
Hands-on: 1.5 hours
Total: 6 hours
Platform compatability
Illumina
Target Genes
Targets mutation hotspots across 17 genes most frequently mutated in lung cancers
AKT1
MET
ALK
NRAS
BRAF
PIK3CA
EGFR
RET
ERBB2
ROS1
ESR1
STK11
HRAS
TP53*
KEAP1
KRAS
MAP2K1
*Whole coding region coverage (NM_000546)
Benefits
Capture more of your patient sample with ATOM-Seq which captures all single stranded, double-stranded or nicked DNA of any length.
Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations
Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases
Unique Dual Indexes
Unique Dual Indexes (UDIs) are required to be purchased and are sold separately
Please contact us to discuss additional index options.
Resources
Product guide and protocol
For Research Use Only (RUO).
Not for use in diagnostic procedures. Not intended to be used for treatment of human or animal diseases.