NEXT-GENERATION SEQUENCING TECHNOLOGY
MethylRich-ATOMseq
Unlocking DNA methylation insights through targeted enrichment of CpG-rich regions.
A streamlined approach combining DNA conversion, CpG-directed capture and ATOM-Seq library preparation to make methylation-focused sequencing more accessible from limited DNA samples.
TECHNOLOGY OVERVIEW
More methylation information from precious samples
DNA methylation is an important epigenetic signal in cancer biology. Yet comprehensive methylation sequencing can be costly, particularly when DNA input is limited. MethylRich-ATOMseq is designed to enrich CpG-containing DNA fragments before next-generation sequencing, concentrating sequencing effort on methylation-informative regions.
01 / FOCUSED
CpG-rich region enrichment
CG-directed capture primers preferentially recover converted DNA containing CpG sites, helping focus the resulting library on informative regions.
02 / INFORMATIVE
Base-resolution methylation readout
Bisulfite or enzymatic conversion supports sequence-based assessment of methylation status at individual cytosine positions.
03 / EFFICIENT
Designed for cost efficiency
Enrichment aims to reduce the sequencing burden compared with whole-genome approaches, subject to assay design and validation.
HOW IT WORKS
A simple, fast workflow
.png)
The workflow integrates methylation conversion with selective CpG capture and ATOM-Seq-based library construction.
1. Start with cell-free DNA
Isolate fragmented DNA, including cfDNA from liquid biopsy samples.
2. Convert DNA
Apply bisulfite or an appropriate enzymatic conversion method to preserve methylation information in the sequence readout.
3. Capture CpG-containing fragments
Two rounds of CG-directed primer annealing and extension enrich selected CpG-containing molecules
4. Add sequencing adaptors
Use ATOM-Seq adaptor-template oligonucleotide chemistry to introduce library adaptor sequences.
5. Amplify and sequence
Complete library amplification and analyse methylation patterns by NGS.