XCeloSeq Colon Cancer cfDNA Kit V2
®
NGS Library Preparation kit for use with cell-free DNA from liquid biopsy
Home > ATOM-Seq Capture Chemistry > Cell-free DNA Enrichment Workflow > XCeloSeq Colon Cancer cfDNA Kit V2
Use ATOM-Seq to capture cell-free DNA and enrich for mutation hotspots across 22 genes frequently mutated in colon cancer and 5 important DPYD SNPs for detection of the even the rarest mutations.
Product Details
Specifications
Product Code
SEQ031
Gene Targets
23
Targeting Primers
Pool 1: 155, Pool 2: 156
Supported Input Material
Cell-free DNA
Recommended Input Quantity
Recommended: 30 - 50 ng
Minimum: 1 ng
Larger quantities will improve maximum sensitivity
Required time
Hands-on: 1.5 hours
Total: 6 hours
Platform compatability
Illumina
Target Genes
Targets mutation hotspots across 22 genes most frequently mutated in colon cancers and DPYD
AMER1
FGFR4
SMAD4
APC
GNAS
TCF7L2
ARAF
HRAS
TP53*
BRAF
KRAS
CTNNB1
MAP2K1
DMD
MAP2K2
DPYD
NRAS
EP300
PDGFRA
ERBB3
PIK3CA
FBXW7
ROS1
*Whole coding region coverage (NM_000546)
DPYD variants rs67376798, DPYD*A2 (rs3918290), rs55886062 and HapB3 (rs75017182, rs56038477) are covered.
Please contact us to discuss customising this panel.
Read about all our customisation options here.
Benefits
Capture more of your patient sample with ATOM-Seq which captures all single stranded, double-stranded or nicked DNA of any length.
Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations
Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases
Unique Dual Indexes
Unique Dual Indexes (UDIs) are required to be purchased and are sold separately
Please contact us to discuss additional index options.
Resources
Product guide and protocol
For Research Use Only (RUO).
Not for use in diagnostic procedures. Not intended to be used for treatment of human or animal diseases.