Polymerase Chain Displacement Reaction

At least 10-fold increased sensitivity than conventional PCR because PCDR produces twice as much material in each round of thermo-cycling:

 

• Traditional PCR uses a pair of primers that generate no more than two-fold amplification per cycle

• For some viruses with frequent mutations a single pair of primers will not be sufficient to identify all variants

• PCDR uses more than one pair of primers and generates up to four-fold amplification per cycle, giving benefits of:

  • sensitivity higher than PCR

  • speed is improved over PCR

  • all variants are identified

• PCDR uses conventional PCR platforms