XCeloSeq® Targeted RNA Enrichment Kits 

NGS Library Preparation kits for cancer detection from FFPE RNA samples

The XCeloSeq Targeted RNA Enrichment Kits generate high quality sequencing libraries for detection of cancer-related gene fusion events from FFPE-preserved material.

 

ENHANCED LIBRARY COMPLEXITY

All XCeloSeq products use the ATOM-Seq® capture chemistry which is built to be uniquely suited for use with FFPE material and liquid biopsy, delivering unrivaled library complexity

FUSION-AGNOSTIC TARGETED ENRICHMENT

 XCeloSeq target enrichment uses a single target-specific primer in the conserved exon of fusion events, allowing for detection of both known and unknown fusion partners

 

STREAMLINED AND FAST WITH HIGHEST SAMPLE RETENTION

XCeloSeq protocols have been designed to be completed within 1 day with the fewest bead purifications and shortest hands-on times

Both known and

unknown gene fusions

Detects:

Protocol Time:

Total: 7h 20m

Hands-on: 1h 55m

Input Amount*:

Recommended: 5-200ng

Minimum: 1ng

The XCeloSeq Targeted RNA Workflow

Using the ATOM-Seq DNA Capture Method, Unique Molecular Identifiers (UMIs) and universal adapter sequences are incorporated directly into available 3' ends of sample cDNA . Then, two rounds of target-specific PCR generate sequencing-ready libraries.

Double Strand cDNA Synthesis

Hands-on Time: 35 minutes

 Incubation Time: 1 hour

Sample RNA is copied to produce double stranded cDNA, allowing interrogation of gene fusions in either orientation.

xceloseq_rna_workflow_1.png
xceloseq_rna_workflow_2.png

ATOM-Seq DNA Capture

Hands-on Time: 10 minutes

 Incubation Time: 1 hour and 45 minutes

cDNA is combined with the Adapter Template Oligo (ATO).

Incorporation of universal priming site and Unique Molecular Indicator directly onto the sample cDNA molecule.

xceloseq_rna_workflow_3.png
xceloseq_rna_workflow_5.png

Two rounds of Nested,

Target-Specific PCR

Hands-on Time: 1 hour and 10 minutes
Incubation Time: 2 hour and 35 minutes

Multiplexed target-specific primers are paired against a universal primer for target enrichment PCR. Using only a single target-specific primer means that both known and novel fusion partners can be enriched for in this way.

A second round of target-specific PCR using a nested primer maximises on-target rate of sequencing, and incorporates dual-index sample barcodes (i5 and i7).

xceloseq_rna_workflow_6.png
RNA_Target_PCR2.png

Sequencing-ready library in 7 hours, 20 minutes.

The XCeloSeq Targeted RNA Enrichment Product Range:

All Targeted RNA Enrichment Kits use a common protocol document, available here.

Please also see the relevant product insert(s) for kit-specific information and protocol steps, given below.

Please note that an XCeloSeq UDI Kit (containing unique premixed i5 and i7 indexing primers) must be purchased separately to use any of the library preparation kits listed on this page. Please see below for more information.

XCeloSeq Fusion Research Kit

A comprehensive panel for detection of known and unknown gene fusion partners for a variety of cancer types, from FFPE-preserved samples.

Product code: SEQ007

Gene Targets: 74

View Product Insert

Reactions per kit: 8

Targeting Primers: 458

View target genes

Please wait - retrieving target list

XCeloSeq Lung Cancer Fusion Kit

A panel for detection of known and unknown gene fusion partners in lung cancer, from FFPE-preserved samples.

Product code: SEQ008

View Product Insert

Gene Targets: 15

Reactions per kit: 8

Targeting Primers: 159

View target genes

Please wait - retrieving target list

XCeloSeq Solid Cancer Fusion Kit

A panel for detection of known and unknown gene fusion partners in solid cancers, from FFPE-preserved samples.

Product code: SEQ012

Gene Targets: 53

View Product Insert

Reactions per kit: 8

Targeting Primers: 352

View target genes

Please wait - retrieving target list

XCeloSeq Sarcoma Fusion Kit

A panel for detection of known and unknown gene fusion partners in soft tissue cancers, from FFPE-preserved samples.

Product code: SEQ014

View Product Insert

Gene Targets: 26

Reactions per kit: 8

Targeting Primers: 146

View target genes

Please wait - retrieving target list

XCeloSeq Actionable Fusion Kit

A panel for detection of actionable gene fusion partners from FFPE-preserved samples.

Product code: SEQ015

Gene Targets: 12

View Product Insert

Reactions per kit: 8

Targeting Primers: 136

View target genes

Please wait - retrieving target list

XCeloSeq Myeloid Fusion Kit

A panel for detection of known and unknown gene fusion partners in myeloid malignancies.

Product code: SEQ017

Gene Targets: 39

View Product Insert

Reactions per kit: 8

Targeting Primers: 310

View target genes

Please wait - retrieving target list

XCeloSeq Lymphoma Fusion Kit

A panel for detection of known and unknown gene fusion partners in lymphoid malignancies.

Product code: SEQ018

View Product Insert

Gene Targets: 33

Reactions per kit: 8

Targeting Primers: 241

View target genes

Please wait - retrieving target list

XCeloSeq Acute Lymphoblastic Leukaemia Fusion Kit

A panel for detection of known and unknown gene fusion partners in acute lymphoblastic leukaemia.

Product code: SEQ019

View Product Insert

Gene Targets: 44

Reactions per kit: 8

Targeting Primers: 317

View target genes

Please wait - retrieving target list

XCeloSeq Unique Dual Indexing Sets (IDX1-01 to IDX1-12)

The XCeloSeq Unique Dual Indexing (UDI) Sets allow for multiplexing of up to 96 different samples each with a unique combination of i5 and i7 indexes for next-generation sequencing on Illumina sequencing platforms. All index oligos undergo rigorous quality control procedures including generation and sequencing of indexed libraries to ensure high quality performance with all compatible XCeloSeq Library Preparation Kits.

When purchasing UDI Sets for use in combination with any of the XCeloSeq Targeted RNA Enrichment Kits up to 96 samples can be multiplexed together when purchasing all 12 Sets. Each UDI combination contains enough for 8 samples, for a total of 128 samples for every UDI Set.

At least one UDI Set must be purchased with XCeloSeq Targeted RNA Enrichment Kits.

Click Here to download product information for ordering, multiplexing, and sample requirments.

*Higher quantities within this range will improve maximum sensitivity, recommended maximum is 200 ng total FFPE RNA or 100 ng high quality RNA. The product supports capture with down to 1.0 ng of RNA, however this is not recommended as it will lead to reduced sensitivity. Cell-free total nucleic acids and cell-free RNA are also supported as alternative starting materials, when using these materials maximising starting material within the above range will help ensure high quality results.

For more information or for sales equniries, please contact us here or at info@genefirst.com