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Adapter Template Oligo-Mediated Sequencing

ATOM-Seq: a unique alternative to ligation-based DNA capture

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ATOM-Seq Technological Overview

ATOM-Seq is a unique approach to NGS library preparation, specifically designed to address the challenges of processing limited or poor-quality clinical samples. 

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The patented ATOM-Seq capture chemistry is simple and elegant. In a polymerase-driven reaction, the 3' end of every 3' end of the sample molecules is extended to directly incorporate true Unique Molecular Identifiers (UMIs) and a universal primer site.

 

This is achieved by annealing sample molecules to a synthetic template known as an Adaptor Template Oligo, which the poylmerase uses as a template to build a 3' extension directly onto the sample molecule without ligation.

Key Benefits

Capture more of your patient sample with ATOM-Seq which captures all single stranded, double-stranded or nicked DNA of any length. 

Better performance with limited samples 

with optimisation to maximise the amount of information which can be obtained from precious patient samples

Process even the most degraded and damaged samples, with resilient workflows able to process even the lowest quality sample.

Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations

Simple, single-day protocols

requiring minimal hands-on time.

XCeloSeq NGS Library Preparation Solutions

ATOM-Seq capture is the foundation of all XCeloSeq Library Preparation Kits

Targeted cfDNA

For identification of single nucleotide variants, insertions, and deletions from limited cell-free DNA

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Compatible with plasma and urine samples​

Whole-sample cfDNA

For capturing all DNA from a sample to identify all genetic and/or epi-genetic signatures from limited cell-free or FFPE-preserved DNA

Targeted FFPE-RNA 

For identification of known and novel gene fusions from poor quality RNA samples such as FFPE-RNA.

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Also allows with detection of single nucleotide variants, insertions, deletions, and relative RNA expression

Targeted FFPE-DNA

For identification of single nucleotide variants, insertions, deletions, copy number variation and MSI from even poor quality FFPE-preserved DNA

Customised solutions

The ATOM-Seq chemistry has solutions to answer almost any clinical or research questions, even beyond those listed above

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If our off-the-shelf products don't match your needs or you are looking for something more unique, please contact us to discuss your project and how we can support you

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