XCeloSeq Breast Cancer cfDNA Kit V2
®
NGS Library Preparation kit for use with cell-free DNA from liquid biopsy
Home > ATOM-Seq Capture Chemistry > Cell-free DNA Enrichment Workflow > XCeloSeq Breast Cancer cfDNA Kit V2
Use ATOM-Seq to capture cell-free DNA and enrich for mutation hotspots across 12 genes frequently mutated in lung cancer for detection of the even the rarest mutations.
Product Details
Specifications
Product Code
SEQ033
Gene Targets
12
Targeting Primers
Pool 1: 122, Pool 2: 119
Supported Input Material
Cell-free DNA
Recommended Input Quantity
Recommended: 30 - 50 ng
Minimum: 1 ng
Larger quantities will improve maximum sensitivity
Required time
Hands-on: 1.5 hours
Total: 6 hours
Platform compatability
Illumina
Target Genes
Targets mutation hotspots across 12 genes most frequently mutated in breast cancers
AKT1
SF3B1
CCND1
TP53*
EGFR
ERBB2
ERBB3
ESR1
FBXW7
FGFR1
KRAS
PIK3CA
*Whole coding region coverage (NM_000546)
Benefits
Capture more of your patient sample with ATOM-Seq which captures all single stranded, double-stranded or nicked DNA of any length.
Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations
Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases
Unique Dual Indexes
Unique Dual Indexes (UDIs) are required to be purchased and are sold separately
Please contact us to discuss additional index options.
Resources
Product guide and protocol
For Research Use Only (RUO).
Not for use in diagnostic procedures. Not intended to be used for treatment of human or animal diseases.