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Total Cell-free DNA Workflows

ATOM-Seq powered NGS Library Preparation kits for capturing all cell-free DNA from liquid biopsies

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The ATOM-Seq total cfDNA capture workflows form the basis of all XCeloSeq cfDNA capture kits and are optimised to capture all nucleic acids from a sample. They generate the highest quality sequencing libraries from liquid biopsy cfDNA samples.

Detect even the rarest clinical signatures by using both UMIs and unique, error-reducing workflow optimisations

Simple, single-day protocols leveraging the inherent simplicity and efficiency of DNA polymerases

XCeloSeq Total cell-free DNA Capture Workflows

Using the ATOM-Seq DNA capture method, Unique Molecular Identifiers (UMIs) and universal adapter sequences are incorporated directly onto available 3' ends of every original sample molecule. Linear amplification then copies all the captured molecules, which are then captured by a second ATOM-Seq capture reaction. Finally, every captured molecule is amplified by global PCR.

Standard workflow

High-GC cfDNA

methylation workflow

Bisulfite conversion

Workflows resistant to DNA damage by using linear amplification to generate new 3' ends, improving performance with poor quality samples 

Maximise retention of cfDNA with bead purifications only after the first PCR amplification step, minimising sample loss

Core Specifications

The total cfDNA and methyl-cfDNA kit workflow times are shown below

* Higher input masses will provide better sensitivities

Sequencing Platform Compatibility

All XCeloSeq products are compatible with all common sequencing platforms.

Please contact us for availability or customisation options.

ComparisonData

ATOM-Seq Advantages for Methylation Analysis

Bisulfite conversion

Processed as above

Workflow Data

The ATOM-Seq cell-free DNA capture workflows were compared. The same input mass of cfDNA was used for both workflows. Both workflows were able to reliably capture cfDNA with little material loss when a bifulfite conversion was included for the methylation workflow.

whole_cfDNA_Workflow_5.png

XCeloSeq Total cfDNA Kits

XCeloSeq cfDNA Kit

XCeloSeq

Methyl-cfDNA kit

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